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SBI
/ Servers / ModLink+: prediction of fold using protein interactions.
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Query Sequence
Enter the query sequence in FASTA format
Query sequence should be pasted in the text area in
FASTA format
. Only
one
sequence should be pasted in the text area.
more...
Clear
Or, upload file
Use the browse button to upload a file from your local disk containing the query sequence. The file should contain only
one
sequence in
FASTA format.
Protein-Protein Interactions
Databases
BIND
BioGRID
DIP
HPRD
IntAct
MINT
MIPS
STRING
Choose the database(s) of protein-protein interactions
Select
any number
of the previous protein-protein interaction databases.
more...
Advanced options
Proteins Interacting with the Query
Enter up to 10 sequences in FASTA format of proteins interacting with the query
Protein(s) interacting with the query (if any is known) should be pasted in the text area in
FASTA format
.
Up to 10
different sequences should be pasted in the text area.
more...
Clear
Nº of proteins
Select the
exact number
of submitted proteins interacting with the query.
10
9
8
7
6
5
4
3
2
1
none
Or, upload file
Use the browse button to upload a file from your local disk containing your protein(s) interacting with the query. The file should contain
up to 10
different sequences in
FASTA format
.
Algorithm Parameters
Combine with
PSI-BLAST
HHSearch
PRC
Choose the extrapolation type
Select the background method to detect similarities in SCOP (
PSI-BLAST
,
HHSearch
or
PRC
).
more...
Extrapolation
by family
by superfamily
by fold
no extrapolation
Choose the extrapolation type
Select the type of extrapolation of links (
by family
,
superfamily
or
fold
) or
no extrapolation
to skip this process.
more...
MHS
no limit
500
100
50
40
30
20
10
5
Choose the maximum hub degree
Select the
maximum hub degree
allowed during the extrapolation process or
no limit
.
more...
EVTE
1e-12
1e-11
1e-10
1e-09
1e-08
1e-07
1e-06
1e-05
1e-04
1e-03
1e-02
1e-01
1
10
100
Choose the threshold
e
-value
Select a
threshold
on the BLAST
e
-value to finish the extrapolation process.
more...
EVTH
don't use
1e-100
1e-90
1e-80
1e-70
1e-60
1e-50
1e-40
1e-30
1e-20
1e-10
1e-09
1e-08
1e-07
1e-06
1e-05
1e-04
1e-03
1e-02
1e-01
1
10
100
Choose the threshold
e
-value
Select a
threshold
on the BLAST
e
-value to assign putative proteins that interact with the query.
more...
PSI-BLAST parameters
Nº of iterations
5
4
3
2
Choose the maximum number iterations
Select the
maximum
number of iterations to search against TrEMBL database to construct the PSSM profile of your query sequence.