blastall arguments: -p Program Name [String] -d Database [String] default = nr -i Query File [File In] default = stdin -e Expectation value (E) [Real] default = 10.0 -m alignment view options: 0 = pairwise, 1 = query-anchored showing identities, 2 = query-anchored no identities, 3 = flat query-anchored, show identities, 4 = flat query-anchored, no identities, 5 = query-anchored no identities and blunt ends, 6 = flat query-anchored, no identities and blunt ends, 7 = XML Blast output, 8 = tab-delimited fields [Integer] default = 0 -o BLAST report Output File [File Out] Optional default = stdout -F Filter query sequence (DUST with blastn, SEG with others) [String] default = T -G Cost to open a gap (zero invokes default behavior) [Integer] default = 0 -E Cost to extend a gap (zero invokes default behavior) [Integer] default = 0 -X X dropoff value for gapped alignment (in bits) (zero invokes default behavior) [Integer] default = 0 -I Show GI's in deflines [T/F] default = F -q Penalty for a nucleotide mismatch (blastn only) [Integer] default = -3 -r Reward for a nucleotide match (blastn only) [Integer] default = 1 -v Number of database sequences to show one-line descriptions for (V) [Integer] default = 500 -b Number of database sequence to show alignments for (B) [Integer] default = 250 -f Threshold for extending hits, default if zero [Integer] default = 0 -g Perfom gapped alignment (not available with tblastx) [T/F] default = T -Q Query Genetic code to use [Integer] default = 1 -D DB Genetic code (for tblast[nx] only) [Integer] default = 1 -a Number of processors to use [Integer] default = 1 -O SeqAlign file [File Out] Optional -J Believe the query defline [T/F] default = F -M Matrix [String] default = BLOSUM62 -W Word size, default if zero [Integer] default = 0 -z Effective length of the database (use zero for the real size) [Real] default = 0 -K Number of best hits from a region to keep (off by default, if used a value of 100 is recommended) [Integer] default = 0 -P 0 for multiple hits 1-pass, 1 for single hit 1-pass, 2 for 2-pass [Integer] default = 0 -Y Effective length of the search space (use zero for the real size) [Real] default = 0 -S Query strands to search against database (for blast[nx], and tblastx). 3 is both, 1 is top, 2 is bottom [Integer] default = 3 -T Produce HTML output [T/F] default = F -l Restrict search of database to list of GI's [String] Optional -U Use lower case filtering of FASTA sequence [T/F] Optional default = F -y Dropoff (X) for blast extensions in bits (0.0 invokes default behavior) [Real] default = 0.0 -Z X dropoff value for final gapped alignment (in bits) [Integer] default = 0 -R PSI-TBLASTN checkpoint file [File In] Optional -n MegaBlast search [T/F] default = F -L Location on query sequence [String] Optional -A Multiple Hits window size (zero for single hit algorithm) [Integer] default = 40 -w Frame shift penalty (OOF algorithm for blastx) [Integer] default = 0 -t Length of the largest intron allowed in tblastn for linking HSPs (0 disables linking) [Integer] default = 0