BLASTP 2.1.3 [Apr-1-2001] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|7920398|gb|AAF70635.1|AF238381_1 PTOV1 [Homo sapiens] (410 letters) Database: /disc9/DB/blast/swissprot 90,939 sequences; 32,775,839 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value sp|P41176|KAS2_STRCM PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE ... 34 0.68 sp|Q99835|SMO_HUMAN SMOOTHENED HOMOLOG PRECURSOR (SMO) 34 0.88 sp|Q02578|KAS1_STRCN PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 1 31 5.0 sp|Q05356|KAS1_STRHA PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE ... 31 7.3 sp|P06489|EXON_HSV2 ALKALINE EXONUCLEASE 30 7.6 >sp|P41176|KAS2_STRCM PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 2 (ORF 2) Length = 402 Score = 34.0 bits (77), Expect = 0.68 Identities = 26/72 (36%), Positives = 31/72 (42%), Gaps = 5/72 (6%) Query: 2 VRPRRAPYRSGAGGPLGGRG---RPPRPLVVRAVRSRSW-PASPRGPQPPRIRARSAPPM 57 V PR AP P GG P R + R + P RG PP RA +AP Sbjct: 188 VEPRSAPGAGSPSSPAGGMSDSDEPNRAYLPFDRDGRGYVPGGGRGVVPPLERAEAAPA- 246 Query: 58 EGARVFGALGPI 69 GA V+G GP+ Sbjct: 247 RGAEVYGEAGPL 258 >sp|Q99835|SMO_HUMAN SMOOTHENED HOMOLOG PRECURSOR (SMO) Length = 787 Score = 33.6 bits (76), Expect = 0.88 Identities = 34/114 (29%), Positives = 44/114 (37%), Gaps = 5/114 (4%) Query: 12 GAGGPLGGRGRPPRPLVVRAVRSRSWPASPRGPQPPRIRARSAPPMEGARVFGALGPIGP 71 G G G P P RS A+ GP PP A P E R LG + P Sbjct: 27 GRGAASSGNATGPGPRSAGGSARRS--AAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLP 84 Query: 72 SSPGLT-LGGLAVSEHRLSNKLLAWSGKRRPYSDSTAKLKRTLPCQAYVNQGEN 124 T L G + S+ KL+ WSG R + + + L C Y+ + EN Sbjct: 85 YGATSTLLAGDSDSQEEAHGKLVLWSGLRN--APRCWAVIQPLLCAVYMPKCEN 136 >sp|Q02578|KAS1_STRCN PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 1 Length = 422 Score = 30.9 bits (69), Expect = 5.0 Identities = 22/68 (32%), Positives = 29/68 (42%), Gaps = 2/68 (2%) Query: 14 GGPLGGRGRPPRPLVVRAVRSRSWPASPRGPQPPRIRARSAPPMEG--ARVFGALGPIGP 71 G +GG R V+ + R + W R +P RA S + A FGA GP+ Sbjct: 105 GSAVGGTTRLEHDYVLVSERGQRWDVDHRAAEPELHRAFSPSTLAADVAERFGAQGPVQT 164 Query: 72 SSPGLTLG 79 S G T G Sbjct: 165 VSTGCTSG 172 >sp|Q05356|KAS1_STRHA PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 1 (KS) (POLYKETIDE CONDENSING ENZYME) Length = 422 Score = 30.5 bits (68), Expect = 7.3 Identities = 25/79 (31%), Positives = 33/79 (41%), Gaps = 4/79 (5%) Query: 5 RRAPYRSGA--GGPLGGRGRPPRPLVVRAVRSRSWPASPRGPQPPRIRARSAPPMEG--A 60 R P+R GA G +GG R V+ + W R +P RA + + A Sbjct: 94 REDPWRMGAVLGTAVGGTTRLEHDYVLVSEGGSRWDVDHRRAEPHLHRAFAPSTLASTVA 153 Query: 61 RVFGALGPIGPSSPGLTLG 79 FGA GP+ S G T G Sbjct: 154 ETFGAQGPVQTVSTGCTSG 172 >sp|P06489|EXON_HSV2 ALKALINE EXONUCLEASE Length = 620 Score = 30.2 bits (67), Expect = 7.6 Identities = 25/62 (40%), Positives = 26/62 (41%), Gaps = 5/62 (8%) Query: 18 GGRGRPPRPLVVRAVRSRSWPASPRGPQP----PRIRARSAPPMEGARVFGALGPIGPSS 73 G R PPRP A S WP SP QP P +AR A P G P P S Sbjct: 38 GPRPAPPRPTSPGAPGSH-WPQSPPRGQPDGGAPGEKARPASPALSEASSGPPTPDIPLS 96 Query: 74 PG 75 PG Sbjct: 97 PG 98 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: Sequences not found previously or not previously below threshold: sp|P41176|KAS2_STRCM PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE ... 34 0.70 sp|Q99835|SMO_HUMAN SMOOTHENED HOMOLOG PRECURSOR (SMO) 34 0.91 sp|Q02578|KAS1_STRCN PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 1 31 5.2 sp|Q05356|KAS1_STRHA PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE ... 30 7.6 sp|P06489|EXON_HSV2 ALKALINE EXONUCLEASE 30 8.2 CONVERGED! >sp|P41176|KAS2_STRCM PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 2 (ORF 2) Length = 402 Score = 34.0 bits (77), Expect = 0.70 Identities = 26/72 (36%), Positives = 31/72 (42%), Gaps = 5/72 (6%) Query: 2 VRPRRAPYRSGAGGPLGGRG---RPPRPLVVRAVRSRSW-PASPRGPQPPRIRARSAPPM 57 V PR AP P GG P R + R + P RG PP RA +AP Sbjct: 188 VEPRSAPGAGSPSSPAGGMSDSDEPNRAYLPFDRDGRGYVPGGGRGVVPPLERAEAAPA- 246 Query: 58 EGARVFGALGPI 69 GA V+G GP+ Sbjct: 247 RGAEVYGEAGPL 258 >sp|Q99835|SMO_HUMAN SMOOTHENED HOMOLOG PRECURSOR (SMO) Length = 787 Score = 33.6 bits (76), Expect = 0.91 Identities = 34/114 (29%), Positives = 44/114 (37%), Gaps = 5/114 (4%) Query: 12 GAGGPLGGRGRPPRPLVVRAVRSRSWPASPRGPQPPRIRARSAPPMEGARVFGALGPIGP 71 G G G P P RS A+ GP PP A P E R LG + P Sbjct: 27 GRGAASSGNATGPGPRSAGGSARRS--AAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLP 84 Query: 72 SSPGLT-LGGLAVSEHRLSNKLLAWSGKRRPYSDSTAKLKRTLPCQAYVNQGEN 124 T L G + S+ KL+ WSG R + + + L C Y+ + EN Sbjct: 85 YGATSTLLAGDSDSQEEAHGKLVLWSGLRN--APRCWAVIQPLLCAVYMPKCEN 136 >sp|Q02578|KAS1_STRCN PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 1 Length = 422 Score = 30.9 bits (69), Expect = 5.2 Identities = 22/68 (32%), Positives = 29/68 (42%), Gaps = 2/68 (2%) Query: 14 GGPLGGRGRPPRPLVVRAVRSRSWPASPRGPQPPRIRARSAPPMEG--ARVFGALGPIGP 71 G +GG R V+ + R + W R +P RA S + A FGA GP+ Sbjct: 105 GSAVGGTTRLEHDYVLVSERGQRWDVDHRAAEPELHRAFSPSTLAADVAERFGAQGPVQT 164 Query: 72 SSPGLTLG 79 S G T G Sbjct: 165 VSTGCTSG 172 >sp|Q05356|KAS1_STRHA PUTATIVE POLYKETIDE BETA-KETOACYL SYNTHASE 1 (KS) (POLYKETIDE CONDENSING ENZYME) Length = 422 Score = 30.5 bits (68), Expect = 7.6 Identities = 25/79 (31%), Positives = 33/79 (41%), Gaps = 4/79 (5%) Query: 5 RRAPYRSGA--GGPLGGRGRPPRPLVVRAVRSRSWPASPRGPQPPRIRARSAPPMEG--A 60 R P+R GA G +GG R V+ + W R +P RA + + A Sbjct: 94 REDPWRMGAVLGTAVGGTTRLEHDYVLVSEGGSRWDVDHRRAEPHLHRAFAPSTLASTVA 153 Query: 61 RVFGALGPIGPSSPGLTLG 79 FGA GP+ S G T G Sbjct: 154 ETFGAQGPVQTVSTGCTSG 172 >sp|P06489|EXON_HSV2 ALKALINE EXONUCLEASE Length = 620 Score = 30.1 bits (67), Expect = 8.2 Identities = 25/62 (40%), Positives = 26/62 (41%), Gaps = 5/62 (8%) Query: 18 GGRGRPPRPLVVRAVRSRSWPASPRGPQP----PRIRARSAPPMEGARVFGALGPIGPSS 73 G R PPRP A S WP SP QP P +AR A P G P P S Sbjct: 38 GPRPAPPRPTSPGAPGSH-WPQSPPRGQPDGGAPGEKARPASPALSEASSGPPTPDIPLS 96 Query: 74 PG 75 PG Sbjct: 97 PG 98 Database: /disc9/DB/blast/swissprot Posted date: Dec 19, 2000 1:52 PM Number of letters in database: 32,775,839 Number of sequences in database: 90,939 Lambda K H 0.322 0.139 0.431 Lambda K H 0.267 0.0425 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 57083150 Number of Sequences: 90939 Number of extensions: 2584341 Number of successful extensions: 8652 Number of sequences better than 10.0: 140 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 140 Number of HSP's that attempted gapping in prelim test: 8004 Number of HSP's gapped (non-prelim): 652 length of query: 410 length of database: 32,775,839 effective HSP length: 48 effective length of query: 362 effective length of database: 28,410,767 effective search space: 10284697654 effective search space used: 10284697654 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 66 (30.0 bits)